Which diagnostic test is not used for diagnosing Burkholderia in humans?

The Mallein Test is traditionally used for diagnosing glanders, which is caused by the bacterium Burkholderia mallei. While Burkholderia species can be pathogenic, the Mallein Test specifically targets infections associated with Burkholderia mallei and is not relevant for diagnosing infections caused by other Burkholderia species, like Burkholderia pseudomallei, which causes melioidosis.

In contrast, serological assays, culture methods, and polymerase chain reaction (PCR) are all effective diagnostic tools for identifying Burkholderia species in humans. Serological assays can help detect antibodies against the bacteria, culture methods allow for the growth and identification of the bacteria from clinical samples, and PCR provides a rapid and specific way to detect the genetic material of the pathogen. Therefore, the Mallein Test does not apply to the diagnosis of Burkholderia in humans in a broader context outside of glanders.